![]() Adult stem cells are currently the only type of stem cells commonly used to treat human diseases Serving as a sort of repair system for the body, they can theoretically divide without limit (self renewal) to replenish other cells as long as the person is still alive. Stem cells have the remarkable potential to develop into many different cell types (self differentiation) in the body. This data can be analyzed to provide further information about subpopulations within the sample. The data are collected and stored in the computer. The characteristics or parameters of each event are based on its light scattering and fluorescent properties. Any suspended particle of cell from 0.2-150 micrometers in size is suitable for analysis. In the flow cytometer, particles are carried to the laser intercept in a fluid stream. Measuring CD34 +cell viability allows us the quality of control assessments of stem cells processing.įlow cytometry, stem cells, CD34+ antigen, cord blood, white blood cells, viabilityįlow cytometer system analyze biological specimen in order to identify various cell populations determined by the specific monoclonal antibodies and fluorochromes used.Ī flow cytometer is made up of three main systems: fluidics, transports particles in a stream to the laser beam for interrogation, optics, consists of lasers to illuminate the particles in the sample stream and optical filters to direct the resulting light signals to the appropriate detectors, and electronics, converts the detected light signals into electronic signals that can be processed by the computer. Conclusion: cryopreservation and processing of autologous stem cells collection, assessing CD34+ cell viability and absolute counts, found to remain the same during periods of time. Viable CD34+ cells were enumerated using single platform flow cytometry and the molecular exclusion dye 7-amino actinomycin D (7- AAD). Results: four-thousands cord blood samples were analyzed for absolute viable CD34+ cells, WBC and their viability. To enhance further our study, post-freeze white blood cells (WBC) counts from an automated haematology analyzer were compared with WBC derived after cytometric analysis. Method: the aim in our study was to prove that time has no effect in CD34+ cells storage, efficacy and viability using flow cytometry as a quantitative single cell analysis. Cord blood samples, has posed additional challenges over the years particularly due to the presence of varying number of nucleated red blood cells (RBC) and the extended age of the samples at the time of CD34+ cells enumeration. Since different cell types can be distinguished by quantization structural features, flow cytometry can be used to count cells of different types in a mixture. It can process measurements on thousands cells in a few seconds. ![]() Background: flow cytometry is a technique of quantitative single cell analysis.
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